5 grams is Terrance McKenna style “heroic” dose.
@bryan_johnson is about to get fucked.
He will not fear death, he will realize that HE is the AI and these bodies are just collecting synthetic data, to cure the boredom.
Doing magic mushrooms this Sunday.
Protocol
+ dose: 5 grams
+ frequency: 1x/mo for 3 mo
+ objective: exploring longevity effects
Measurement
+ 249 independent biomarkers
+ 29 vials of blood
+ brain scans
+ urine, stool, saliva, fertility
+ multi-omics profiling (DNA, epigenetics, metabolism, hormones, microbiome, proteins, cognition)
Cardiovascular
+ LDL-C
+ HDL-C
+ Triglycerides
+ Total cholesterol
+ Apolipoprotein B
+ Apolipoprotein A-1
+ Lipoprotein(a)
+ LDL-P
+ HDL-P
+ Small LDL-P
+ LDL size
+ Large VLDL-P
+ Large HDL-P
+ VLDL size
+ HDL size
+ LP-IR score
+ Oxidized LDL
+ Lp-PLA2 activity
+ Myeloperoxidase (MPO)
+ Homocysteine
+ VEGF
Hematology
+ WBC
+ RBC
+ Hemoglobin
+ Hematocrit
+ MCV
+ MCH
+ MCHC
+ RDW
+ Platelets
+ Neutrophils
+ Lymphocytes
+ Monocytes
+ Eosinophils
+ Basophils
+ Immature granulocytes
+ Erythropoietin (EPO)
+ ABO/Rh typing
Hormonal
+ Thyroid panel with TSH
+ Free T4
+ Testosterone
+ Dihydrotestosterone (DHT)
+ 5α-DHT
+ Prolactin
+ Estradiol
+ LH
+ FSH
+ Estrone (E1)
+ Estriol (E3)
+ Androstenedione
+ Androsterone
+ 5β-Androstanediol
+ Etiocholanolone
+ Cortisol
+ Cortisone
+ DHEA
+ DHEA-S
+ Pregnenolone
+ IGF-1
+ Melatonin (6-hydroxymelatonin sulfate)
Metabolic
+ Glucose
+ Insulin
+ Hemoglobin A1c
+ α-Hydroxybutyrate
+ β-Hydroxybutyrate
+ Uric acid
Kidney
+ BUN
+ Creatinine
+ eGFR
+ BUN/Creatinine ratio
+ Cystatin C
+ Urinalysis (specific gravity, pH, color, appearance, leukocyte esterase, protein, glucose, ketones, occult blood, bilirubin, urobilinogen, nitrite, WBC [hpf], RBC [hpf], epithelial cells, casts, bacteria)
+ Albumin/Creatinine ratio
Pancreatic
+ Total protein
+ Albumin
+ Globulin
+ Total bilirubin
+ Alkaline phosphatase
+ AST
+ ALT
+ GGT
+ Amylase
+ Lipase
Minerals
+ Sodium
+ Potassium
+ Chloride
+ CO₂ (bicarbonate)
+ Calcium
+ Magnesium
+ Iron
+ TIBC
+ UIBC
+ Iron saturation
+ Ferritin
+ Zinc
Vitamins
+ Vitamin B12
+ 25-hydroxy Vitamin D
+ Coenzyme Q10
+ α-Tocopherol
+ β-Carotene
+ Glutathione
+ Total glutathione
Amino Acids
+ Cysteine
+ Methionine
+ Taurine
+ Glycine
+ Serine
+ Threonine
+ Alanine
+ Valine
+ Leucine
+ Isoleucine
+ Phenylalanine
+ Tyrosine
+ Tryptophan
Inflammation
+ Interleukin-6 (IL-6)
+ Interleukin-10 (IL-10)
+ Tumor Necrosis Factor-α (TNF-α)
+ Rheumatoid factor (RF)
+ ANA by IFA
+ Calprotectin (GI inflammation),
+ Secretory IgA (mucosal immunity)
Neurology
+ P-tau217
+ Epinephrine metabolites (metanephrine, normetanephrine)
+ Homovanillate (HVA)
+ Vanilmandelate (VMA)
+ 5-Hydroxyindoleacetate (5-HIAA)
Toxicology
+ Lead
+ Lithium
Urology
+ PSA total
Fertility
+ Total Motile Count
+ Sample Volume
+ Concentration
+ Motility
+ Morphology
+ Count
Metabolomics
+ Citrate
+ cis-Aconitate
+ Isocitrate
+ α-Ketoglutarate
+ Succinate
+ Fumarate
+ Malate
+ Pyruvate
+ Lactate
+ 2-Hydroxyglutarate,
+ Hydroxymethylglutarate,
+ 3-Methylglutaconate,
+ 2-Methylsuccinate
+ Formiminoglutamate (FIGLU),
+ Methylmalonate (MMA),
+ Xanthurenate
+ Kynurenate,
+ Quinolinic acid
+ Glucarate,
+ 8-Hydroxy-2′-deoxyguanosine (8-OHdG),
+ Pyroglutamate
+ 3-Hydroxyphenylacetate,
+ 4-Hydroxyphenylacetate,
+ 2-Hydroxyphenylacetic acid
+ 4-Cresol,
+ p-Hydroxybenzoate,
+ Benzoate,
+ Hippurate/Hippuric acid
+ Phenylacetate/Phenylacetic acid,
+ Indoleacetate/Indoleacetic acid
+ Arabinose,
+ Citramalic acid,
+ α-Hydroxyisobutyric acid
Microbiome
+ Bacteroides fragilis
+ Bacteroides vulgatus
+ Faecalibacterium prausnitzii
+ Akkermansia muciniphila
+ Roseburia spp.
+ Eubacterium rectale
+ Lactobacillus spp.
+ Bifidobacterium spp.
+ Escherichia coli
+ Clostridium difficile
+ Enterococcus faecalis
+ Candida albicans
+ Saccharomyces boulardii
+ Prevotella copri
+ Ruminococcus spp.
+ Methanobrevibacter smithii
+ Blautia spp.
+ Proteobacteria spp.
+ Firmicutes/Bacteroidetes ratio
+ Beta-glucuronidase
+ Butyrate
+ Propionate
+ Acetate
Aging & Epigenetics
+ Telomere length
+ Relative telomerase activity
+ DNA methylation age
+ DNA methylation levels at CpG sites associated with biological aging
+ Glucocorticoid receptor methylation (NR3C1)
+ SIRT1 methylation
+ MTOR methylation
+ Oxidative stress response genes (NFE2L2 promoter methylation)
Cognitive
+ Brain age score
+ Functional connectivity strength
+ Global connectivity index
+ Hemodynamic response amplitude
+ Hemodynamic response latency
+ Resting-state oxygenated hemoglobin (HbO) concentration
+ Resting-state deoxygenated hemoglobin (HbR) concentration
+ Total hemoglobin (HbT) concentration
+ Oxygen saturation (O₂Sat)
+ Cerebral blood volume (CBV)
+ Cerebral blood flow (CBF)
+ Neurovascular coupling index
+ Prefrontal cortex oxygenation
+ Parietal cortex oxygenation
+ Temporal cortex oxygenation
+ Occipital cortex oxygenation
+ Task-evoked hemodynamic response (HbO/HbR)
+ Temporal autocorrelation of hemodynamics
+ Functional brain network modularity
+ Inter-regional coherence
+ Brain lateralization index
+ Oscillatory power (slow cortical fluctuations)
+ Phase synchronization index
+ Mean reaction time (during cognitive tasks)
+ Reaction time variability
+ Heart-rate variability (HRV)
+ Resting heart rate